麻黄汤及拆方对哮喘小鼠5-脂质氧合酶激活蛋白、白介素4基因的表达和白三烯C_4的影响

Effects of among compositions of Herba Ephedrae decoction on genic xpression of 5-lipoxygenase activating protein,IL-4 and leukotriene C_4 in asthmatic mice

目的:通过对麻黄汤(Herba Ephedrae decoction,HED)各拆方配伍组对哮喘小鼠5-脂质氧合酶激活蛋白(FLAP)、白介素4(IL-4)基因的表达和白三烯C4(LTC4)的影响来探讨其配伍规律。方法:建立小鼠卵蛋白哮喘模型,除正常对照组和模型组外其余各组分别灌胃给予麻黄汤及拆方(以麻黄生药剂量2 500 mg.kg-1)和阳性药地塞米松(2 mg.kg-1),连续用药7 d后用实时荧光定量PCR方法检测肺组织中5-脂质氧合酶激活蛋白、白介素4mRNA表达的变化,用ELISA方法检测支气管肺泡灌洗液中LTC4含量。结果:哮喘小鼠肺组织中FLAP,IL-4基因表达水平、支气管肺泡灌洗液(BALF)中LTC4水平均较正常对照组显著升高。麻黄汤及拆方组可以不同程度抑制小鼠肺组织中FLAP,IL-4基因表达水平,BALF中LTC4水平。结论:麻黄汤具有明显抗过敏哮喘的作用,拆方分析显示麻黄汤全方效果最佳,从分子水平验证了麻黄汤组方的科学性和合理性。

Objective： To explore the regularity of recipe composition by observing inhibitory effects on the genic expression of 5-lipexygenase activating protein, IL-4 and the leukotriene CA in asthmatic mice. Method： The mice were challenged with OVA and administered ig with the Herba Ephedrae decoction （HED）, separated compositions （2 500 mg·kg^-1, calculated by Herba Ephedrae） and dexamethasone （2 mg·kg^-1 ） respectively once daily for seven days. The real-tree fluorescence quantitative PCR method was employed to measure the contents of FLAP mRNA and IL-4 mRNA expressions in lung and the ELISA method was used to determine the content of LTC4 in the washing solution of pulmonary alveolus and bronchi. Result： In the lung of asthma mice, the expressions of FLAP and IL-4 and the content of LTC4 were significantly augmented compared with the control group. The HED and the separated compositions could suppress the expressions of FLAP and IL-4 and LTC4 release to a great extent in mice. Conclusion： The HED had the remarkable effects of antianaphylaxis asthma and the original formula HED worked best. TheSe results confirmed the rationality and scientific level of HED.