摘要
目的:在超声条件下寻找提取连钱草总黄酮的优化条件。方法:采用正交设计法,以超声功率、超声时间、提取温度、溶剂体积分数为因素,每个因素3个水平,选择L9(34)正交表,用紫外分光光度法测定总黄酮的含量做为评价指标。结果:最佳提取条件为超声功率800W,超声时间90(45,45)min,提取温度40℃,溶剂浓度65%。最佳提取条件下总黄酮的质量分数为5.228%。结论:以乙醇为溶剂,采用超声循环提取技术提取连钱草中总黄酮,提取温度低,时间短,提取效率高,具有广阔前景。
Objective： To investigate the optimum condition of extraction for the flavonoids in Glechoma longituba by ultrasonic wave. Method： Using orthogonal test, the effects of ultrasonic power, ultrasonic time, extraction temperature and solvent concentration were considered, the comprehensive evaluation was guided by the content of the flavonoids determined by ultraviolet spectrophotometer. Result： The optimum condition was as follow： ultrasonic power; 800 W, ultrasonic time 90 min, extraction temperature 40 ℃, solvent concentration 65%. The flavonoids concent is 5. 228%. Conclusion： Using ethanol as solvent, circulated extraction of the flavonoids from G. longituba with ultrasonic wave is feasible with the optimum conditions in low temperature, short time and high production yield.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2007年第5期385-387,共3页
China Journal of Chinese Materia Medica
关键词
连钱草
黄酮
超声循环提取
正交试验
Glechoma longituba
flavonoids
ultrasonic and circulated extraction
orthogonal test
