X-5大孔树脂纯化新乌头碱和乌头总碱

Study on separation and purification of total alkaloids and mesaconitine with X-5 macroporous resin

目的:对影响X-5树脂分离纯化乌头总生物碱和新乌头碱的各种因素进行系统的研究,使分离工艺达到最优化。方法:以川乌总碱和新乌头碱的含量为指标,考察X-5型大孔树脂分离纯化乌头总生物碱和新乌头碱的最佳吸附及洗脱条件。结果:X-5树脂纯化乌头总生物碱和新乌头碱的最佳工艺为上样液浓度含生药1g.mL-1,药液pH 12,吸附时间为6h,7BV pH8的95%乙醇洗脱效果好。X-5树脂可重复使用5次。经X-5树脂处理乌头总碱提取率可达80%以上,终产品总生物碱纯度可达30%以上。结论:该方法能明显提高新乌头碱和乌头总碱的纯度。

Objective： To study systematically the factors which affect separation and purification of the total alkaloids and mesaconitine with X - 5 macroporeus resin. Method： With the content of the total alkaloids and mesaconitine as parameters, the optimum condition of absorption and elution were studied in the process of the purification with X -5 macroporeus resin. Result： The X - 5 macreporeus resin yielded the best separating efficiency when the concentration of the extracted solution was lg raw material per lmL, pH 12. 0 , the absorptive time of 6 hour and the volume of 95% ethanol （TBV pH 8） as the eluant; X -5 macroporeus resins was used five times in a reproducible way. The rate of extraction and content of the total alkaloid were 80% and 30% respectively after purification with X- 5 macroprous resin. Conclusion： The method can increase the purity of mesaconitine and total alkaloids.