红花等10种中药的植物雌激素活性研究

Evaluation on phytoestrogen effects of ten kinds of Chinese medicine including flos carthami

目的:评价红花、川牛膝、丹参、女贞子、枸杞、甘草、肉苁蓉、淫羊藿、补骨脂、菟丝子等10味中药的植物雌激素活性。方法:选用昆明种雌性乳鼠,随机分为A,B二组:A组又分为12组(溶剂对照组、己烯雌酚组和10个中药组);B组也分为12组(溶剂对照组、己烯雌酚组和10个中药拮抗组),其中中药拮抗组每天同时灌胃中药和己烯雌酚;各组给药4d后分离血清。利用雌激素受体(ER)阳性MCF-7细胞,以MTT法检测中药含药血清对细胞增殖的影响。结果:A组中,红花、川牛膝、丹参、枸杞、肉苁蓉、淫羊藿、补骨脂和菟丝子等中药含药血清可显著促进MCF-7细胞增殖;而女贞子组可见细胞增殖受到明显抑制。B组中,红花、川牛膝、丹参、甘草、淫羊藿、补骨脂和菟丝子组可见己烯雌酚促细胞增殖的作用受到了显著抑制;而肉苁蓉可协同并显著增强己烯雌酚促细胞增殖的作用。结论:红花、川牛膝、丹参、淫羊藿、补骨脂、菟丝子等6种中药在单独用药时具有拟雌激素作用,在与雌激素同时用药时具有抗雌激素作用,其双向调节效应依赖于体内雌激素水平的高低。

Objective： To explore the phytoestrogenic effects of ten kinds of Chinese medicine including flos carthami, radix cyathulae, radix salviae miltiorrhizae, fructus lignstri lucidi, fructus lycii, radix clycyrrhizae, herba cistanches, herba epimedii, fructus psoraleae and semen cuscutae. Method： 240 female Kunming mice weighting 9 - 12 g were randomly divided into two main groups A and B. A group was divided into 12 small groups： 1 solvent control group, 1 diethylstilbestrol control group and 10 Chinese medicine groups. B group was also divided into 12 small groups： 1 solvent control group, 1 diethylstilbestrol control group and 10 Chinese medicine antagonistic groups. Mice in ten antagonistic groups were administered beth Chinese medicine and diethylstilbestrol everyday. After administered（op） for 4 days, blood was collected and serum was separated. The effect of the pharmacological serum on proliferation rate of MCF-7 （ER ＋ ） was analyzed by MTr-assay. Result： In A group, proliferation rates of MCF-7 cells treated with serum from eight Chinese medicine groups including flos carthami, radix cyathulae, radix salviae miltiorrhizae, fructus lycii, herba cistanches, herba epimedii, fructus psoraleae and semen cuscutae were coued markedly increase respectively. While serum from fructus ligustri lucidi group could markedly decrease the proliferation rate of MCF-7 cells. In B group, the increased proliferation rate of MCF-7 cells caused by diethylstilbestrol was significantly reduced in seven Chinese medicine antagonistic groups including flos carthami, radix cya- thulae, radix salviae miltiorrhizae, radix clycyrrhizae, herba epimedii, fructus psoraleae and semen cuscutae. While the increased proliferation rate could be markedly enhanced in herba cistanches group. Conclusion： Six kinds of Chinese medicine such as flos carthami, radix cyathulae, radix salviae miltiorrhizae, herba epimedii, fructus psoraleae and semen cuscutae show beth estrogenic effects （ when administered indepently） and antiestrogenic effects （when administered together with diethylstilbestrol ）. Such bidirectional effects depends on the internal estrogen level.