新疆哈萨克族人群中血脂水平与AT_1R基因A1166C多态性的相关研究

The Relationship between Dyslipemia and Polymorphism of AT_1R Gene A1166C in Kazakans of Xinjiang

目的研究新疆哈萨克族人群血脂异常与血管紧张素Ⅱ1型受体基因(AT1R)A1166C多态性之间的相关性。方法采用多聚酶链式反应法及限制性片段长度多态性技术(PCR-RFLP),对新疆哈萨克族296例原发性高血压(EH)患者及198例正常血压对照者的外周血白细胞DNA,进行AT1R基因A1166C多态性检测,并用全自动生化检测仪测定所有研究对象的各项血脂生化指标。结果AA、AC和CC基因型及A、C等位基因在高血压人群和对照人群中分布差异无统计学意义(P>0.05)。但总胆固醇和低密度脂蛋白水平在高血压人群中高于正常对照人群,两者差异有统计学意义(P<0.001)。同时,在高血压人群中偏相关分析结果显示总胆固醇和低密度脂蛋白水平与基因型存在着相关性,即AC基因型携带者总胆固醇和低密度脂蛋白水平高于AA基因型携带者。结论AT1R基因A1166C分子变异与新疆哈萨克族原发性高血压无相关关系。但在高血压人群中总胆固醇和低密度脂蛋白水平与AT1R基因A1166C多态位点分子变异存在着相关性。

Objective To investigate the relationship between dyslipemia and polymorphism of angiotensin Ⅱ type Ⅰ receptor （AT1R） gene A1166C in Kazakans of Xinjiang. Methods Polymerase chain reaction and restriction fragment length polymorphism （PCR- RFLP） methods were used to detect the A1166C polymorphism of AT1R gene in Kazakans including 296 patients with hypertension and 198 normotensive controls. Biochemical indexes were detected by autochemical hemalyzer. Results The genotype frequencies of A1166C and the A1166/1166C allele frequencies were not statistic significances in hypertension group compared with normotensive group （ P 〉 0.05）. But the TC and LDL in hypertension group are higher than those in the normotensive group （ P 〈 0.001）. In hypertension group, TC and LDL are related to A1166C polymorphism of AT1R gene. TC and LDL in AC genotype group are higher than those in AA genotrpe group. Conclusions There was no significant association found between the A1166C polymorphism of AT1R gene and essential hypertension in Kazakans of Xinjiang. But the dyslipemia is related to A1166C polymorphism of AT1R gene in essential hypertension group of Kazakans.