肺癌细胞核形态测定及DNA定量分析

NUCLEAR MORPHOMETRY AND DNA CYTOMETRY IN PULMONARY CARCINOMA

①目的探讨肺癌细胞核形态参数及ＤＮＡ含量与肿瘤生物学行为的关系。②方法应用图象分析技术对８２例原发性肺癌标本进行细胞核形态和ＤＮＡ原位定量测定。③结果在肺腺癌，癌细胞ＤＮＡ含量、核面积、核周长及核形态因子与肿瘤大小、有无淋巴结转移及病人预后均有明显关系，且肿瘤长径＞３ｃｍ组、淋巴结癌转移组的ＤＮＡ六倍体（６Ｃ）以上病例数，均显著多于肿瘤长径≤３ｃｍ组及淋巴结癌转移阴性组（χ２＝７．８３，５．２５；Ｐ均＜０．０５）；以上指标均与肺鳞癌无关。④结论细胞核形态和ＤＮＡ测定对于估测肺腺癌病人的预后有重要意义；

Objective To study the relationship between DNA content, nuclear morphometric protocols and biological behavior of pulmonary carcinoma. Method Nuclear morphometry and DNA cytometry were performed with image analysis technique in 82 cases of pulmonary carcinoma. Result In pulmonary adenocarcinoma, DNA content and morphometric data were remarkably correlated with the size of the neoplasm, the lymph node metastasis and the prognosis of patients. The contents of DNA 6C was significantly higher in tumors with diameters greater than 3cm and in those with lymph node metastasis( χ 2=7.83,5.25;P <0 05). In squamous cell carcinoma, no obvious correlation was observed between morphometric data, DNA content and the biological behavior of the tumor. Conclusion In pulmonary adenocarcinoma, nuclear morphometry and DNA cytometry are important values for predicting the patients prognosis; DNA 6C can be used for determining the malignancy of pulmonary adenocarcinoma.