摘要
通过正交试验设计,确定适合嗜热链球菌最佳冻干保护剂配方为脱脂乳粉12%,海藻糖1%,甘油3%,谷氨酸钠1%(均为质量分数)。通过加速储存实验,同批冻干样品在不同温度(30,42,60℃)条件下储存,分别采样,测定活菌数,建立储存稳定性方程,由方程外推出得到4℃降解速率常数为1.656×10-4。
The optimum cryoprotectants formula for S. thermophilus was determined by the orthogonal trial as：nonfat milk powder 12%, tre- halose 1%, glycerol 3%, sodium glutamine 1%. The stability equation during storage Was established by the accelerated storage test. Samples were incubated at 30,42 and 60 ℃. Viability assays were performed on each sample removed at constant intervals of time. The specific rate of degradation at 4 ℃ was estimated as 1.656×10^-4 by the equation.
出处
《中国乳品工业》
CAS
北大核心
2007年第2期8-10,26,共4页
China Dairy Industry
基金
国家863计划项目(2002AA248041)
关键词
乳酸菌
冷冻干燥
保护剂
加速储存
lactic acid bacteria
freeze drying
cryoprotectants
accelerated storage test
