ELISA法检测牛奶中链霉素残留量

Determination of streptomycin residues in milk by ELISA

从抗原的制备、抗体的获得、酶结合物的合成等基础入手,建立了牛奶中链霉素残留量快速筛选检测方法,检测历时60min,方法的临界值为110μg/L。经试验证明该方法检测限可达20μg/L或以下,奶样用水稀释后可直接测定,批内变异系数小于20%,板间变异系数小于30%,200μg/L质量浓度处空白奶样添加回收率在60%-110%,符合快速筛选方法的要求。

ELISA is developed to detect streptomycin residues in milk after the p＇roduction of antigen, antibody and enzyme Conjugate. I takes 60 minutes to complete the whole assay operation. The detection limit and cut-off value of this method are 30 μg/L and 110 μg/L respec tivety. Sa.mple, after diluted by water, could be analyzed directly. The intra artd inter coeffcient variation are with in 20% and 30% respectively.