检测P53在宫颈癌中的表达及临床意义

The expression of 53 in cervical carcinoma

目的检测P53在宫颈癌中的表达,探讨P53在宫颈癌发生发展中所起的作用。评估其作为肿瘤标志物及临床预后指标的价值。方法收集宫颈鳞状上皮癌石蜡包埋标本51例、慢性宫颈炎石蜡包埋标本21例,另收集子宫肌瘤全宫术后正常宫颈组织石蜡包埋标本13例作为正常对照组,采用免疫组化检测三组中的P53的蛋白表达,分析P53在宫颈癌中的蛋白表达强度与病理分级、淋巴转移、病理分型等的关系,分析P53与宫颈癌发生发展的关系。结果P53在正常对照组中的表达分别为12例阴性(-)、1例弱阳性(+),阳性表达率为7.69%;在慢性宫颈炎中18例阴性(-)、3例弱阳性(+),阳性表达率为14.29%;51例宫颈癌组织中共为43例阳性,阳性表达率为84.31%,分别为8例阴性(-)、17例弱阳性(+)、12例阳性(++)、14例强阳性(+++)。P53在宫颈癌组织中表达显著性均高于炎症组和对照组(P<0.01),P53的表达量与病理分级呈明显正相关(P<0.01),与病理分型、淋巴转移无明显关系(P>0.05)。结论P53与宫颈癌的发生发展密切相关。

Objective To evaluate the expression and clinical significance of tumor suppressor gene P53 in the progression of cervical carcinoma. Methods Fifty-one patients with cervical carcinoma and twenty-one patients with chronic cervicitis and 13 cases of normal cervical epithelium in people after total hysterectomy were investigated immunhistochemically for expression of P53 in paraffin sections from 1995 to 2000. Results The expressions of P53 in normal epithelium were twelve cases of negative, one of weak positive; the positive proportion was 7.69%. The expressions of P53 in chronic cervicitis group was eighteen cases of negative, three of weak positive, respectively, and the positive proportion was 14.29%. The expression of P53 in cervical carcinoma groups was eight of negative, seventeen of weak positive, twelve of positive, and fourteen of strong positive. The positive proportion was 84.31%. The expressions of P53 in cervical carcinoma group with grade Ⅰ , Ⅱ, and Ⅲ were 19, 22, 10, respectively. There was significant difference of expression of P53 between cervical carcinoma group and chronic cervicitis group or controls group （P〈 0.01 ）. Conclusion P53 is associated with the progression of cervical carcinoma.