间接光度色谱法测定血定安溶液中钠、钙、镁的含量

Determination of Sodium. Magnesium and Calcium in Gelofusine by indirect Photometric Chromatography

本文用间接光度色谱法（ＩＰＣ）测定了血定安溶液（Ｇｅｌｏｆｕｓｉｎｅ）中钠、钙及镁的含量。采用ＳｐｈｅｒｉｓｏｒｂＳＣＸ色谱柱（５μｍ，２５０ｍｍ×３ｍｍ），以Ｃｅ３＋为流动相平衡离子，检测波长２５４ｎｍ。样品中各离子的回收率为：Ｎａ＋９８．７％～１００．４％，Ｃａ２＋９８．１％～１０１．７％，Ｍｇ２＋９９．４％～１０４．２％。Ｃａ２＋、Ｍｇ２＋最小检出量分别为：３．２ｎｇ和２．０ｎｇ。本法准确、灵敏。

This paper reported the determination of Na (Ⅰ) 、Mg (Ⅱ) and Ca (Ⅱ) in gelofusine by indirect photometric chromatography (IPC). The column was packed with spherisorb SCX (5μm, 250mm×3mml . The mobile phase counterion was Ce (Ⅲ) . Detection was carried out at a wavelength of 254nm. The recoveries of ions for assay of gelofusine were Na (Ⅰ) 98. 7% ～ 100. 4%, Mg (Ⅱ) 99. 4%～104. 2%, Ca (Ⅱ) 98. 1%～101. 7%. The detection limits of Mg (Ⅱ) and Ca (Ⅱ) were 2. 0ng and 3. 2ng respectively. The IPC method is specific, sensitive, simple and rapid.