芸芥体细胞胚胎发生及植株再生体系的建立

Somatic Embryogenesis and Establishment of Plant Regeneration System of Eruca sativa Mill.

以芸芥子叶为外植体,诱导芸芥体细胞胚胎发生并建立植株再生体系.结果表明:基因型及植物生长调节剂对芸芥体细胞胚胎发生均有一定的影响,其中以含有1.0mg·L-12,4-D的MS培养基诱导芸芥体细胞胚胎发生的效果最优.在MS+0.2mg·L-12,4-D培养基上,胚性愈伤组织可大量增殖.对芸芥体细胞胚胎成熟的研究表明,体胚在N6培养基上成熟最佳,且45.2%的成熟体胚可在1/2MS+0.1mg·L-1IBA培养基上萌发生长.

Cotyledon explants of E. sativa were cultured to induce somatic embryogenesis（SEM） and then the somatic embryos were developed into seedlings. The results revealed that E. sativa genotypes and plant growth regulation agents（PGRs） had different effects on somatic embryogenesis, and the best medium to induce somatic embryogenesis of E. sativa was Murashige and Skoog （MS） medium supplemented with 1. 0 mg · L^-1 2,4-D. Secondary somatic embryos were reproduced on MS medium containing 0.2 mg · L^-1 2, 4-D. Maturation of somatic embryos showed that the somatic embryos were well developed and could differentiate into mature embryos on N6 medium. The mature somatic embryos were germinated and 45.2 of them could develope into plantlets by culturing on 1/2MS medium containing 0.1 mg · L^-1 IBA.