免疫介导的运动神经元损伤SMI-31表达的特征

The SMI-31 expression of motor neuron in immune-mediated injury.

目的观察实验性自身免疫性灰质病模型中上、下运动神经元磷酸化神经丝表达的特征。方法首先建立实验性自身免疫性灰质病模型,然后,通过免疫组化方法研究免疫介导的神经元损伤过程中运动神经元磷酸化神经丝(SMI-31)表达的变化。结果在免疫介导的神经元损伤过程中,大脑皮层及脊髓前角SMI-31免疫反应阳性的神经元数量与对照组相比明显增加(P=0.000)。结论神经丝重链的异常磷酸化可能在在免疫介导的神经元损伤过程中具有重要的作用。

Objective To observe the expression of phosphorylated neurofilaments of the upper and lower motor neurons in experimental autoimmune gray matter disease （EAGMD） animal model. Methods EAGMD animal model was developed by inoculated guinae pig with bovine spinal cord anterior horn homogenate, and using immunohistoehemieal （IHC） method, the expression of SMI-31 in motor neuron were observed. Results The numbers of SMI-31 immunoreactive positive spinal cord （SC） motor neuron and cerebral cortex pyramidal cell in EAGMD were obviously increased （P = 0. 000） than those in controls. Conclusions Abnormal phosphorylation of neurofilament heavy chain （NF-H） may play important role in immune-mediated upper and lower motor neuron injury.