应用PAP法检测成人皮肤切创纤维连接蛋白推断损伤时间

A STUDY ON HTE FIBRONECTIN FORMED IN INCISED WOUND IN ADULTS WITH PAP METHOD AND TIMING OF INJURY

应用PAP法，对15例成人腹部不同时间组（2小时以内）手术切创皮肤进行研究，观察Fn的分布、含量变化及其与损伤时间的关系；同时作H．E和Masson三色染色对比观察。结果发现：创伤即刻，创壁仅有少量Fn阳性颗粒；15分钟后，创壁表面Fn含量增多，呈薄层、断续的细条状；以后，随着时间延长，创壁表面Fn含量逐渐增多，呈连续细带状；至2小时，创伤局部Fn含量明显增多，创壁表面Fn是较宽带状，创壁内Fn亦增多，并是浓度梯度改变。PAP法检测Fn，可为鉴别生前与死后损伤、推断早期损伤时间，提供新的手段。

15 samples of operated incised wound specimen taken randomly from adult abdominal skin at different intervals within 2h after surgery were examined with PAP method. The results indicated that the changes of fibronectin (Fn) in incised wounds were related to the time of injury. At the time immediately after the surgery,only a few positive Fn granules appeared on the wall of the wound. After15min,the amount of Fn increased and distributed on the wound wall and was arranged as an uncontinued strip. At the interval of 30min to 1.5h after insision, more Fn was observed as a continuedstrip. At 2h after insision,the amount of Fn increased significantly and was arranged as a broader stripon the wound wall. Besides, more Fn appeared in the shallow layer than in the deep layer of thewound. The results suggested that detection of Fn with PAP method is of great value for estimatingthe tirne of injury.