双向凝胶电泳的实验操作及进展

Process of Two-dimensional Electrophresis and Its Progress

双向聚丙烯酰胺凝胶电泳是研究蛋白质组的核心技术,本文重点介绍了双向凝胶电泳的原理、实验操作过程中的具体步骤以及近年来在实验过程中发展的一些新方法和进展。

Two-dimensional polyacrylamide gel electrophoresis （2D-PAGE） is a key technique for proteome research. This article have summarized the technic principle, the process of experiment manipulate and the new methods and progress in the process of experiment which had developed recently of Two-dimensional electrophoresis.