剪股颖愈伤组织诱导与植株再生

Study on Callus Induction and Plantlet Regeneration of Creeping Bentgrass(Agrostis palustris Huds.)

以匍匐剪股颖的成熟种子为外植体,对其愈伤组织诱导及再生体系进行了研究。结果表明:愈伤组织诱导合适的培养基为MS+6mg.L-1 2,4-D+0.2mg.L-1 TDZ+500mg.L-1 CH,诱导率达到68.1%;MS+5mg.L-1 2,4-D+0.1 mg.L-1TDZ+500mg.L-1 CH为愈伤组织继代较合适的培养基;愈伤组织分化的合适培养基为MS+0.3mg.L-1 TDZ+0.5mg.L-1 6-BA,分化率达到52.7%。随着愈伤组织继代次数的增加,胚性愈伤组织的分化能力没有明显的降低,这可为后续的遗传转化长期提供受体材料。

Taking mature seeds as explant, the callus induction and plant regeneration system of Agrostis palustris Huds.was optimized.The result showed that the suitable medium for callus induction was basic MS medium,supplemented with 6 mg·L^-1 2,4-D,0.2mg·L^-1 TDZ and 500mg·L^-1 casein hydrolysate,and the ratio of callus induction was 68.1%.The better medium for callus subculture was basic MS medium,supplemented with 5 mg·L^-1 2,4-D,0.1 mg·L^-1 TDZ and 500 mg·L^-1 casein hydrolysate.The basic MS medium contained 0.3 mg·L^-1 TDZ and 0.5 mg·L^-1 6-BA was suitable for differentiation medium, and the differentiation rate was 52.7%. With subculture times increased ,embryogenic callus still keep a high differentiation frequency,it could provide plant material for gene transfer for a long term.