信号肽及其在蛋白质表达中的应用

Signal Sequence and Its Application to Protein Expression

分子生物学研究已进入后基因组时代,其中心任务是更多地关注基因组表达的蛋白质的结构和功能。由于基因功能最终通过其表达产物——蛋白质来实现,因此,要了解基因组全部功能活动,最终也必须回到蛋白质上。另外,在菌株、培养和发酵等逐渐成熟的条件下,构建高效的表达载体以提高外源蛋白质的表达量是降低工业化生产成本的关键。随着研究的深入,发现信号肽对蛋白质的定位有着非常重要的作用,使得信号肽的研究不仅具有重要的理论意义,而且也具有潜在的应用价值。就信号肽的结构和功能,信号肽的捕获方法及其在原核表达系统和真核表达系统中表达外源蛋白质的应用做一些介绍。

The study of molecule biology is coming to terminal genome times now. The important aim is to pay attention to the structures and functions of proteins. The functions of gene were implemented by expression productions -proteins, so if want to know the functions and activities of genome,we must study proteins first. On the other side,when strains ,culture and ferment conditions are mature, then make up of effective expression vectors is the key of reduce the cost of industrial manufacture. Peptides play an important role in the orientation of proteins in pace of further study. Because of these,the study of peptides is not only important in theory,but also worth in applications. Signal peptides＇ structures and functions,signal sequence traps and the proteins expressions in prokaryote and eukaryote expression systems were illustrated in the article.