摘要
目的:建立控制感冒灵胶囊中野菊花的含量测定方法;方法:以蒙花苷为对照品,采用高效液相色谱法进行含量测定。色谱柱:十八烷基硅烷键合硅胶为填充剂(romasil15-C18,250mm×4.6mm,5μm);流动相:甲醇-4%冰醋酸水(49:51);流速:1ml/min;检测波长:334am.结果:蒙花苷在0.1848~1.386μg的范围内,其进样量与峰面积呈良好的线性关系。蒙花苷的平均加样回收率为99.46%,RSD=1.75%;结论:该结果准确,方法重复性好,可作为该制剂的含量测定及质量控制。
Objective: To establish a determinative method for controlling the content of wild chrysanthemum in Coldrine Capsule. Method: To use linarin as control article, HPLC method was used for its determination. The chromatographic conditions included romasil15 -C18 (250 mm × 4.6 mm,5μm) and the mobile phase consisted of methanol -4% glacial acetic acid(49 : 51 ) with the flow rate 1 ml/min; the detection wavelength was 334 nm. Result: When linarin ranged from 0. 1848 μg to 1. 386 μg, there was a good linearity relationship between sample size of linarin and peak area. The average spotting recovery rate of Linarin was 99.64%, RSD = 1. 75% ; Conclusion: The result is accurate, the method has good reproducibility, can be used to control the quality and determinate the content of this preparation.
出处
《中医药导报》
2007年第2期70-71,共2页
Guiding Journal of Traditional Chinese Medicine and Pharmacy