套式聚合酶链反应在母婴传播巨细胞病毒研究中的应用

Application of nested polymerase chain reaction in the study of HCMV maternal-fetal transmission

本文建立了套式ＰＣＲ技术加限制酶分析方法，对１０５份母脐血配对进行套式ＰＣＲ，病毒分离，特异性ＩｇＭ及ＩｇＡ测定。结果母血ＨＣＭＶＤＮＡ阳性６份，脐血３份，母－脐传播率为３／６。三对被证实为母婴传播ＨＣＭＶ套式的标本中，二对套式ＰＣＲ、病毒分离、特异性ＩｇＭ及ＩｇＡ均阳性。一对套式ＰＣＲ病毒分离、特异性ＩｇＡ阳性，但特异性ＩｇＭ阴性，提示：套式ＰＣＲ是一种敏感、特异、简便快速，能早期诊断ＨＣＭＶ的检测手段，适用于孕妇及胎儿ＨＣＭＶ感染的监测。

Nested PCR was developed to detect conserved sequence from the immediate early gene of human cytomegalovirus ( HCMV). 105 pairs of maternal-infant umbilical blood were analysed by nested PCR,virus isolation and specific antibodies.The results indicated that 6 samples of maternal blood were positive with HCMV DNA, 3matched umbilical blood were positive also.The rate of maternal-fetal transmission was 3/6.Of the 3 matched pair blood samples considered as HCMV intrauterine infection,2 pairs were HCMV positive with nested PCR , virus isolation, specific IgM and IgA.One pair was nested PCR,virus isolation, specific IgA positive and specific IgM negative. All these suggest that nested PCR,being sensitive,specific, rapid and simple, is available for the detection of HCMV in pregnant women and fetuses.