红曲促进实验大鼠骨折愈合的骨组织形态计量学观察

The Morphometric Study of Bony Tissue in the Rat Experiment of Bone Union Enhanced by the Red Rice

[目的]探讨红曲治疗实验大鼠骨折的疗效。[方法]将64只Wistar大鼠制成前臂单骨折模型,随机分为红曲治疗组与生理盐水对照组。并于骨折后21d与42d,行骨组织形态学定性观察及骨组织形态计量学观察。[结果]红曲治疗后,明显加速了大鼠骨折愈合,骨组织形态计量学诸指标优于对照组,两组间差异具有统计学意义。红曲能促进骨折愈合后期骨痂的改建,促进板层骨的提早出现和髓腔提前再通。红曲组矿化骨痂面积、提高成骨细胞指数及促进基质的钙化明显大于对照组,P<0.05。[结论]中药红曲可以促进骨痂生长、提高成骨细胞指数、促进基质的钙化、加速骨痂改建从而促进骨折愈合。

[Objective] To investigate the curative effect of the red rice in treating the experimental fractures in the rats. [Methods] 64 Wistar rats suffered with single fracture in the forearm were randomized into two groups, one red rice group and the other normal saline control group. On the twenty-first day and 42 day after operation, the qualitation and morphometric observation of the bone tissue were performed. [Results] After the treatment of the red rice, the bony union of the fractured bone in the rats was obviously accelerated. The morphometric index of the bony tissue was higher than the control group with the statistical significance. The remodeling of the bony callus, the emergence of the lamellar bone and recanalization of the medullary cavity could all be enhanced by the use of the red rice. The mineralized bony callus area, the osteoblast index and the matrical calcification were obviously higher than those of the control group. [Conclusions] The growth of bony callus, the index of the osteoblast, the metrical calcification and the remodeling of the bony callus could all be enhanced by the red rice and therefore the bone union could be accelerated.