冰茶栓含药血清对HEPG2细胞的增殖抑制作用

To Detect the Inhibiting Capacity of Bingcha Shuan Contained Serum to HEPG2 Cells' Reproductive Activity (MTT Method)

[目的]观察冰茶栓含药血清对体外培养的肝癌细胞HEPG2增殖的抑制作用。[方法]用不同冰茶栓剂量组的药物血清对体外培养的HEPG2细胞干预24h、48h,用MTT法来观察冰茶栓含药血清对HEPG2细胞增殖的抑制作用。[结果]冰茶栓含药血清对HEPG2细胞增殖具有一定的抑制作用。且这种作用呈时间、剂量依赖性,随着剂量的增加和时间的延长,抑制率增高。[结论]冰茶栓具有抑制肝癌细胞增殖的作用,为临床抗肝癌治疗提供实验依据。

[Objective]To observe the inhibiting capacity of Bingcha Shuan Containing serum to HEPG2 cells＇ reproductive activity and to conjecture. Bingcha Shuan has the potential to cure the liver carcinoma. [Method] According to serum pharmacology, HEPG2 cells were cultured in vitro and interfered by various concentrations of Bingcha Shuan contained serum for 24,48 hours respectively. The inhibiting capacity of the Bingcha Shuan contained Serum to HEPG2 cells were detected by MTT. [Result] The Bingcha Shuan contained Serum has the inhibiting capacity to HEPG2 cells in some degree. And there was a marked positive correlation between drug concentration, time and inhibition ratio. [Conclusion] It is suggested that Bingcha Shuan has the inhibiting capacity of cell proliferation and it can provide the support on how to cure the liver cancer of clinic.