助孕方对免疫性不孕雌性大鼠的疗效观察

Therapeutic Observation on Female Rats in Immune Sterility Treated by Zhuyun Decoction

[目的]观察助孕方对免疫性不孕大鼠的疗效影响。[方法]设立生理盐水组、模型组、助孕方组、强的松组,各组分别以精子、福氏佐剂等混悬液腹股沟皮下注射免疫雌性SD大鼠造模,生理盐水(NS)组皮下注射NS。造模4周后,生理盐水组、模型组服用NS,助孕方组、强的松组分别服用助孕方药和强的松,2周后与雄鼠合笼。在首次免疫前、各次加强免疫后1周取血ELISA法测定AsAb,SABC法检测子宫内膜IgG、IgA免疫积分,同时比较各组大鼠受孕数及孕鼠平均着床点数。[结果]助孕方组着床点数及受孕数明显高于其他各组,助孕方能降低子宫内膜IgG、IgA积分。[结论]助孕方对免疫性不孕大鼠具有一定的疗效,其机制可能与其降低子宫内膜IgG、IgA免疫积分的免疫调节有关。

[Objective] To observe the effect of Zhuyun Decoction on immune sterility rats. [Method] Set up groups of normal saline, model, Zhuyun Decoction and prednisone, make models of every group by injecting subcutaneously suspension of sperm and Fu＇s adjuvant, etc. into female SD rats along the groin, NS into normal saline group. After 4 weeks, the groups of normal saline and model take NS, Zhuyun Decoction for Zhuyun Decoction group, prednisone for prednisone group. After 2 weeks, they are closed together with male ones. Before the first immune and 1 week after strengthened immune, take blood and measure AsAb with ELISA method, measure the immune integral of IgG and IgA in uterus tunica intima with SABC method; meanwhile, compare the pregnant rats and average nidation points in all groups. [Result] In the Zhuyun Decoction group, the pregnant rats and nidation points are much more that other groups; Zhuyun Decoction can reduce IgG and IgA integral in uterus tunica intima. [Conslusion] Zhuyun Decoction has definite effect on immune sterility rats, whose mechanism may be related with regulation of IgG and IgA integral in uterus tunica intima.