Bcl-2反义寡核苷酸联合照射对肺癌细胞的抑制作用

Inhibition of Bcl-2 Antisense Oligonucleotide Combined with Radiation against Lung Carcinoma Cells

[目的]研究Bcl-2反义寡核苷酸联合照射对肺癌细胞的抑制作用。[方法]NCI-H446肺癌细胞株分5组:空白对照、单纯照射组、反义寡核苷酸加照射组、无义序列加照射组、脂质体加照射组。相应组照射10GY后24、48、72小时,MTT法检测各组的细胞增殖抑制率和联合相互作用。[结果]照射组、反义寡核苷酸加照射组、无义序列加照射组、脂质体加照射组均有细胞抑制,并呈时间依赖关系。其中Bcl-2反义寡核苷酸联合照射组抑制率明显高于其它三组(P<0.01),联合作用指数(CDI)=0.86。[结论]Bcl-2反义寡核苷酸促进了照射对NCI-H446肺癌细胞的增殖抑制作用,两者有协同作用。

[Objective]To study the inhibitory effect of bcl-2 antisense ollgonucleotide （ASODN） combined with radiation on lung carcinoma cells NCI-H446. [Method]The cultured NCLH446 lung carcinoma cells were divided into 5 groups： control, pure radiation, nonsense＋ radiation, lipofectin＋ radiation, ASODN＋ radiation. Every group except for control were assayed by MTT on the inhibition rates,24h,48h,72h after 10Gy irradiation respectively. [Results]The inhibitions of lung carcinoma cells NCI-H446 were observed in ASODN＋ radiation, lipofectin＋ radiation, nonsense＋ radiation and pure radiation groups. The differences between the ASODN＋ radiation group and the other three groups were significant（P%0.01）. [Conclution]The results suggest that combination of ASODN and radiation can enhance the inhibitory effects on the proliferation of lung carcinoma cells NCI-H446.