γ-生育三烯酚抑制人胃癌SGC-7901细胞增殖及促凋亡作用

γ-Tocotrienol inhibits cell proliferation of human gastric carcinoma SGC-7901 cells and induces apoptosis

目的为了研究γ-生育三烯酚（γ-tocotrienol，γ-T3）对人胃癌细胞SGC-7901细胞的增殖抑制作用。方法采用细胞生长曲线，细胞核分裂，集落形成，DNA合成，Western blotting等方法，观察了不同剂量γ-T3（0，15，30和60umol/L）对SGC-7901细胞增殖及其相关细胞凋亡蛋白表达的影响。结果γ-T3可明显抑制SGC-7901细胞增殖；细胞核分裂、集落形成、DNA合成的抑制率24h分别为13．54％．77．02％、73．30％．98．36％和16．16％．65．33％；48h分别为14．15％-89．14％、90．7％-98．92％和35．1％-81．89％；Western blotting方法研究果表明，γ-T3可抑制Bcl-2蛋白表达，促进Bax蛋白表达，并诱导caspase-3蛋白产生17KD的活化片断。结论γ-T3对SGC-7901细胞生长有明显抑制作用，其抑制机制与参与调节Bcl家族相关蛋白表达有关。

Objective To investigate the effect of apoptosis on human gastric carcinoma SGC-7901 cells induced by γ- tocotrienol.Methods SGC-7901 cells were treated with different concentrations of γ-tocotrienol（ 15,30 and 60 umol/L）, with a negative control. The curve of cell growth, cellular mitosis, clone formatting, DNA synthesis and western blotting for correlated protein expression of apoptosis were employed. Results γ-Tocotrienol inhibited the cell proliferation. The inhibitory frequencies （IF, % ） of cellular mitosis, clone formatting and DNA synthesis were 13.54% - 77.02%, 73.30% - 98.36% and 16.16% - 65.33% at 24 h, 14.15% - 89.14% ,90.7% - 98.92% and 35.1% - 81.89% at 48 h, respectively. The further study showed γ-Tocotrienol inhibited the expression of Bcl-2 and promoted the expression of Bax, and induced the cleaved caspase-3 formation. Conclusion γ-Tocotrienol could inhibit SC, C-7901 cells prohferation. Its inhibitory mechanism may participate in adjusting the associated protein expressions of Bcls family.