骨桥蛋白在不同大肠癌细胞株中的转移相关功能研究

Metastasis-related function of osteopontin between two different colorectal cancer cell lines

目的:探索骨桥蛋白(OPN)在大肠癌转移中的相关功能。方法:分别构建OPN反义和正义真核表达质粒后,转染入Colo205和SW480大肠癌细胞株。经鉴定后,进一步运用流式细胞仪、免疫组化、同质和异质黏附等技术检测转染细胞的转移相关功能变化。结果:OPN高表达的大肠癌细胞,CD44表达量增强,E-cadherin的表达减弱;细胞之间同质黏附减弱,细胞侵袭运动能力增强,细胞与ECV304细胞之间的异质黏附作用增强。均证明了OPN与侵袭转移的密切相关性。结论:提示OPN影响大肠癌细胞同质黏附和异质黏附能力的作用可能是大肠癌肝转移发生发展的相关机制之一。

AIM： To analyze the metastasis - related function of osteopontin （OPN） in colorectal cancer cell lines. METHODS： The sense - and antisense -osteopontin eukaryotic expression plasmids were transfected into Colo205 and SW480 cell lines. The metastatic function was detected by flow cytometry, immunohistochemistry, homogeneous and heterogeneous adhesion in different cell lines. RESULTS： High expression of OPN reduced E - cadherin expression and enhanced CD44v6 expression in colorectal cancer cells. Homogeneous adhesion was weakened, but heterogeneous adhesion was enhanced among these cells. CD44v6 expression was intensified to accelerate colorectal cancer cell adhering with ECM and invading into blood vessels and liver. CONCLUSION： OPN is one of potential and important factors in the process of colorectal cancer liver metastasis.