超声微泡介导转染FKBP12.6基因对小鼠H9c2(2-1)心肌细胞中Ca^(2+)浓度的影响

Effects of FKBP12.6 gene on intracellular Ca^(2+) concentration in mouse H9c2 (2-1) myocardial cells transfected by ultrasound-mediated destruction of microbubbles

目的:探讨超声微泡介导转染FKBP12.6基因后,对小鼠H9c2(2-1)心肌细胞中Ca2+浓度的影响。方法:将pcDNA3.1-FKBP12.6质粒与白蛋白包裹微泡造影剂混合,经超声转染H9c2(2-1)细胞后,通过倒置显微镜观察心肌细胞生长状况的变化;激光共聚焦显微镜检测细胞内Ca2+浓度的变化;免疫组织化学方法检测FKBP12.6蛋白的表达。结果:超声触发微泡破裂转染的FKBP12.6基因可在心肌细胞高效表达,细胞生长良好。高表达FKBP12.6的心肌细胞中,总的钙离子浓度增加。结论:超声微泡介导FKBP12.6基因转染心肌细胞,可以明显增加心肌细胞中的Ca2+浓度,心肌细胞的收缩能力增强。

AIM ： To investigate the changes of intracellular calcium ion （ Ca^2＋ ） concentration in mouse H9c2 （2 - 1 ） cells transfected with or without FKS06 binding protein 12.6（FKBP12.6） gene by ultrasound mediated destruction of microbubbles. METHODS ： The pcDNA3.1 - FKBP12.6 plasmid, mingled with albumin - coated microbubbles agents, was transfected into H9c2 （ 2 - 1 ） ceils by ultrasound - mediated destruction of microbubbles. The H9c2 （ 2 - 1 ） cell growth state was investigated by inverted microscope. The changes of intracellular Ca^2＋ concentration was determined by laser scanning confocal microscope. The FKBP12.6 protein expression was checked by immunohistochemistry. RESULTS： As compared with control cells, the H9c2 （2 -1 ） ceils, transfected with FKBP12.6 gene, grew better, had higher gross intracellular Ca^2＋ concentration. CONCLUSION： FKBP12.6 gene augments Ca^2＋ concentration in mouse H9c2 （2 - 1 ） cells, enhances the contractibility of the myocardial cell, which may be helpful to improve the myocardial dysfunction.