雌激素对人骨髓间质细胞BMP-2 mRNA表达的影响

Effects of estrogen on bone morphogenetic protein-2 gene transcription in human bone mesenchymal stem cells

目的:探讨雌激素对人骨髓间质干细胞BMP-2基因转录的影响。方法:将骨髓间质干细胞第3代细胞经1周成骨诱导后,分为17β-雌二醇100nmol/L处理组和对照组,通过RT-PCR法扩增BMP-2 mRNA,测定扩增条带的吸光度值,并与3-磷酸甘油醛脱氢酶看家基因GAPDH的mRNA之比值表示产物mRNA的相对含量。结果:雌激素处理组BMP-2 mRNA表达明显高于非处理组(P<0.05)。结论:雌激素通过刺激间质细胞BMP-2mRNA的表达以发挥其促成骨作用。

AIM： To investigate the effects of estrogen on bone morphogenetic protein -2 gene transcription in human bone mesenchymal stem cells. METHODS： Primary cultures of MSCs were established from normal adult volunteer iliac crest bone marrow aspirates. The third passage of MSCs was supplemented with 50 mg/L ascorbic acid, 10 mmol/L β -glycerophosphate, and 10 nmol/L dexamethasone, to induce MSCs osteogenic differentiation. At day 8, the cultures were treated with 100 nmol/L 17β - estradiol. The expression of bone morphogenetic protein -2 mRNA was detected by reverse transcription （RT - PCR）, the ratio of the electrophoretic results of the BMP -2 mRNA with the housekeeping gene of glyceraldehydes - 3 - phosphate dehydrogenase （GAPDH） mRNA were used to show the relative content of the mRNA. RESULTS： Bone marrow mesenchymal stem cells obtained from human expressed BMP - 2 mRNA as shown by RT - PCR. After 24 h of 100 nmol/L E2 treatment, MSCs BMP -2 mRNA levels increased significantly. CONCLUSION： Estrogen may enhance bone formation by increasing the transcription of BMP - 2 gene of MSCs.