聚乙二醇牛血红蛋白偶联物对血清炎性因子的影响

Effect of Polyethylene Glycol Conjugated Bovine Hemoglobin on Inflammatory Factors During Extracorporeal Circulation in Rabbit

目的探讨在体外循环(extracorporeal circulation,ECC)预充液中加入聚乙二醇牛血红蛋白偶联物(PEG-bHb)对兔血清炎性因子的影响。方法24只成年健康大耳白兔,通过股动脉、颈静脉插管建立体外循环通路,转流1h。随机分成三组,对照组(n=8),以晶/胶体液作为ECC预充液;实验组1(n=8),在预充液中加入PEG-bHb,PEG-bHb占总容量(兔循环血量+预充量)的5%;实验组2(n=8),预充液中加入PEG-bHb,占总容量(兔循环血量+预充量)的15%,ECC开始时放出自体血(放血量占兔循环血量的20%),ECC结束后将自体血回输。每组监测ECC前、中和ECC后的血气参数,用ELISA法测定各时间点血清白介素-6(IL-6)、肿瘤坏死因子(TNF-α)、C反应蛋白(CRP)浓度。结果三组在ECC后各时间点的炎性因子无显著差异,但实验组2在ECC后各时间点的炎性指标较对照组相比有下降的趋势。结论血液代用品PEG-bHb单纯用于预充对兔ECC后炎性反应无影响,用PEG-bHb置换自体血有可能减轻兔ECC后炎性反应。

OBJECTIVE To investigate the effect of PEG - conjugated bovine hemoglobin （ PEG - bHb） on inflammatory factors when added directly to extracorporeal circulation（ECC） prime in anaesthetized rabbit. METHODS 24 anaesthetized rabbits （2.59 ± 0.19 ） kg, connected to ECC by jugular venous and femoral arterial cannulation and bypass for 1 hour, were randomly divided into 3 groups. In control group（n =8）, a crystallold/colloid solution was used as prime solution. In experimental group 1 （ n = 8 ）, PEG - bHb （5 % total volume） was added to the pump prime of bypass. Total volume is the sum of circulation volume and prime volume. In experimental group 2（ n = 8）, PEG - bHb （ 15% total volume） was used in the pump prime with previous blood harvesting（20% circulation volume） before ECC. Blood gas were monitored throughout the procedure. The inflammatory factors （IL -6 ,TNF -α, CRP） were measured using ELISA. RESULTS There were no significant difference of IL - 6, TNF - α and CRP between three groups. But in experimental group 2, the inflammatory factors were less than those in control group, but not significantly. CONCLUSION PEG - bHb added to the prime of a rabbit ECC circuit does not independently affect inflammatory response. PEG - bHb on the prime with previous blood harvesting before ECC may improve inflammatory response.