热休克蛋白70对供心一氧化氮及一氧化氮合酶表达的影响

Effects of Heat Shock Protein 70 on Myocardial Expression of Nitric Oxide and Nitric Oxide Synthase of Donor Heart

目的探讨热休克蛋白70(HSP70)对供心一氧化氮(NO)、一氧化氮合酶(NOS)的影响。方法W istar大鼠18只,分为2组:对照组(C,n=9),腹腔注射生理盐水0.5ml,24h后取离体心脏灌注康斯特保护液(HTK液),4℃保存3h后建立Langendorff离体心脏灌注模型,灌注KH液2h;实验组(E,n=9)腹腔注射重酒石酸去甲肾上腺素(溶于生理盐水中)3.1μmol/kg(0.53mg/kg),腹腔注射24h后取离体心脏,处理方法同C组。测定心肌HSP70含量、NO、NOS的含量以及相关生化指标并做统计学处理比较。结果HSP70含量E组较C组明显增高(P<0.01),NO、NOS的含量E组较C组明显增多(P<0.01),生化指标E组明显优于C组。结论心肌HSP70高表达对供心具有明显的保护效应,并且其促进心肌NO、NOS的表达,可能是HSP70发挥心肌保护作用的因素之一。

OBJECTIVE To investigate the protection of heat shock protein 70 on donor heart and the effects on expression of myocardial cell nitric oxide （NO） and nitric oxide synthase （NOS） of isolated rat heart. METHODS 18 Wistar rats were randomly divided into 2 groups. Control group （ C,n = 9） ,Normal saline 0.5ml was injected intraperitoneally and 24 hours later isolated hearts were stored in 4 ℃ for 3 hours with Histidine - tryptophan - ketoglutarate （HTK） solutions, and then isolated hearts were perfused for2 hours by Langendorff model. Experimental group （E,n = 9）, Noradrenaline Bitartrate 3. 1μmol/kg（0.53mg/kg） was injected intraperitoneally and 24 hours later isolated hearts were stored in 4 ℃ for 3 hours with HTK, and then isolated hearts were perfused for 2 hours with Krebs - Henseleit （ K - H） solutions by Langendorff model. Myocardial HSP70 content , the expression of NO,NOS and related biochemical indicators were detected. RESULTS HSP70 , NO and NOS in E group are higher than those in C group and biochemical indicators in E group is better than that in C group . CONCLUSION This study demonstrated that the high expression of HSP70 has obvious protection on donor heart ,and it can markedly affect the expression of NO ,NOS of isolated rat heart and it is perhaps one of important factors for myocardial protection.