摘要
应用自行制备的直径15nm的胶体金标记SPA,建立了检测血清流行性出血热病毒抗体的滴金免疫测定法。与同步建立的ELISA法对比检测临床标本,结果有良好的一致性,两法符合率为96.4%。本法中抗原、抗体通过渗滤在膜上进行反应,数分种内即可用肉眼观察结果,操作简单,试剂稳定、安全。
The dot immunogold filtration assay by SPA labelled with a colloidal gold particles of 15nm in diameter was established for the detection of viral antibodies in epidemic hemorrhagic fever. A comparison was made in parallel with ELISA established under the same conditions for the clinical speciemens. The results showed that there was a good agreement between these two methods with a coincidence rate of 96.4%. Because the antigen-antibody reaction took place on the membrane during the filtration process, the experimental results co'uld be read with the naked eyes within a few minutes, indicating that this method proved to be quite simple and the reagents were rather stable and safe.
出处
《上海免疫学杂志》
CSCD
北大核心
1996年第3期158-160,共3页
Shanghai Journal of Immunology
关键词
流行性出血热
EHFV
抗体
滴金免疫测定法
SPA-gold probe, anti-epidemic, hemorrhagic fever virus,dot immunogold filtration, ELISA