鼠痘病毒CC强毒株的分离与鉴定

Isolation and identification of CC virulent strain of mouse poxvirus

目的通过病毒分离和分子生物学特性研究,查明在实验小鼠中是否存在鼠痘。方法应用Vero细胞对出现皮疹、丘斑、皮肤溃烂、结痂、组织坏死脱落等疑似鼠痘症状的实验昆明小鼠进行病毒分离,并对其进行形态学、理化学、人工感染试验和分子病毒学等鉴定。结果成功分离出1株鼠痘病毒强毒株,命名为MPVCC株。经PCR扩增和测序鉴定,所分离病毒膜蛋白基因全长333bp,编码111个氨基酸,与MP-2株14ku膜蛋白基因核苷酸序列和推导氨基酸序列的同源性均为100%。结论实验用昆明小鼠中存在鼠痘病毒强毒株感染。

Objective To investigate whether the infection of mouse poxvirus exists in experimental mice, and further study the molecular biological characteristics of isolates, Methods Vero cell line was applied to virus isolation from the mice with skin rash, ulcer, scab and tissue necrosis, which were doubtfully infected with the mouse pox-virus. The isolate was identified by a series of methods such as morphological observation, physicochemistry, artificial challenge and molecular virology. Results A virulent strain CC of mouse poxvirus was successfully isolated from the spleen of a dead Kunming mouse, named as MPV CC strain. By PCR amplification and sequencing, the full length gene of 14 ku membrane protein of CC strain was 333 bp, which encodes 111 amino acids. The nucleotide and amino acid sequences of 14 ku membrane protein of CC strain were compared with MP-2 strain as a reference. The homology was all 100%. Conclusion The infection of virulent strain of mouse poxvirus exists in experimental mice.