胸腺细胞对胸腺止皮细胞分泌IL-6的促进作用

The promoting effect of thymocytes on IL-6 production by an established mouse thymic epithelial cell line (MTEC1)

为分析胸腺细胞对胸腺基质细胞功能的调节作用，将胸腺细胞与小鼠胸腺上皮细胞系（ＭＴＥＣ１）共育，分析胸腺细胞对ＭＴＥＣ１分泌ＩＬ－６的影响。结果表明，胸腺细胞能明显促进ＭＴＥＣ１分泌ＩＬ－６，其促进程度与两种细胞的数量及共育时间有关。胸腺细胞（４×１０＾６／孔）与ＭＴＥＣ１（１×１０＾５／孔）共育４８小时，ＭＴＥＣ１分泌ＩＬ－６达高峰。去除胸腺细胞后９６小时。

To analyse the influence of thymocytes on the function of thymic stromal cells , we examined the effect of fresh thymocytes on IL- 6 production by MTEC1 in coculture of hfTEC1 with thymocytes in vitro. This study provided evidences that thymocytes could significatntly increase IL-6 production by MTEC1 and the effect was in a cell dose and time of cocultured dependent fashion. The maximal effect was observed at 48hr after cocultured thymocytes(4×106/well) with MTE(1×105/well) . After thymocytes were removed from the experimental system , the thymocyte-activated MTEC1 , cultured alone for 96hr , produced 1.7-flod more IL- 6 than MTEC1 without previous interaction with thymocytes. Mitomycin-C- treated thymocytes could still promote IL-6 production by MTEC1 similarly to non-treated cells. Thyrnocytes culture supernatant and lysate had no such effect on IL-6 production by MTEC1. It suggests that thymocytes can promote MTEC1 to produce IL-6 by a mechanism in which direct interaction between thytmocytes and thymic epithelial cells is essential.