宫颈脱落细胞高危型人乳头状病毒核酸扩增微板杂交捕获检测的临床意义

The Clinic Meaning of Cervical Cytological Speciments HPV Micro-plate Hybrid Capture Detection PCR Assays

目的:探讨核酸扩增(PCR)微板杂交捕获检测宫颈脱落细胞内高危型人乳头状病毒(HPV)感染的临床意义,建立快速、灵敏的检测方法。方法:采用PCR微板杂交捕获检测140例宫颈脱落细胞高危型HPV(HPV16/18/31/33/35/39/45/51/52/56/58/59/68)感染状况。结果:细胞学诊断低度鳞状上皮内病变(LSIL)56例,高危型HPVDNA阳性18例(阳性率为32.5%);细胞学诊断高度鳞状上皮内病变(HSIL)42例,高危型HPVDNA阳性25例(阳性率为59.5%);未明确诊断意义的不典型鳞状上皮细胞(ASCUS)31例,高危型HPVDNA阳性11例(阳性率为35.5%);鳞状细胞癌9例,高危型HPV阳性8例(阳性率88.9%)。结论:PCR微板杂交捕获检测宫颈脱落细胞内高危型HPV感染,具有快速、简便、灵敏度高、特异性强等优点,适用于大规模筛查和临床常规工作。

Objective To establish a rapid and sensitive method of detect cervical hisk-risk human papillomavirus infections in cervical cytological speciments. Methods HPV type 16/31/33/35/39/45/51/52/56/58/59/68 DNA were detected in 140 cervical specimens by Micro-plate Hybrid Capture Detection PCR Assys. Results In cervical cytological speciments of 56 LSIL , 18 cases were positive high-risk HPV DNA （32.5%） ;25 cases were positive high-risk HPV DNA in 42 HSIL cytological speciments （59.5 % ） ; Among 31 ASCUS specimens, 11 cases were positive high-risk HPV DNA （ 35.5 % ） ; for the other 9 cervical squmaous cell carcinomas specimens, 8 cases were positive high-risk HPV DNA（88.9% ）. Conclusion Micro-plate Hybrid Capture Detection PCR Assys is a rapid, easy, sensitive and special assay to detect high-risk HPV infection with cervical cytological specimens, it can be applied for large-scale screening and clinical diagnosis.