北京地区正常人群血清中呼吸道合胞病毒F和G蛋白特异性IgG抗体水平的观察

A survey of specific IgG antibodies against respiratory syncytial virus F and G glycoproteins among children and adults in Betjung

以表达呼吸道合胞病毒（ＲＳＶ）Ｆ和Ｇ蛋白的重组痘苗病毒作为抗原，用间接免疫荧光法检测北京地区不同年龄组正常儿童及成人血清中ＲＳＶＦ和Ｇ蛋白特异性ＩｇＧ抗体。其结果表明：成人及产妇静脉血中有中等滴度的ＲＳＶ抗体及较低水平的Ｆ和Ｇ蛋白特异性ＩｇＧ抗体。初生婴儿脐带血中的ＲＳＶＦ和Ｇ蛋白ＩｇＧ抗体水平同母亲静脉血中的一致，表明属母传抗体。婴幼儿ＲＳＶ抗体水平在生后６个月内迅速降低，６个月以后抗体的滴度随年龄消长，７－１４岁时水平最高。Ｆ和Ｇ蛋白特异性ＩｇＧ抗体滴度在生后６个月内无明显降低，但低于大年龄组儿童。６个月后Ｆ和Ｇ蛋白抗体滴度显著升高并随年龄波动，Ｆ较Ｇ蛋白更为显著，分别在３～７岁（Ｆ蛋白）和７－１４岁（Ｇ蛋白）组中达是高滴度。从ＲＳＶ，Ｆ和Ｇ蛋白三种特异性ＩｇＧ抗体间的相关性来看：ＲＳＶ分别同Ｆ和Ｇ蛋白抗体呈现明显的相关性，但Ｆ和Ｇ蛋白特异性ＩｇＧ抗体间无明显相关性。

Serum IgG antibodies specific to respiratory syncytial virus ( RSV ) F and G glycoproteins were determined in children at various ages and adults in Beijing with indirect immunofluorescence technique by using Fang G glycoproteins of RSV respectively expressed by the recombinant vaccinia. The results demonstrated that there were RSV antibody with only moderate titer and low level specific IgG antibodies against F and G glycoproteins in adults. The RSV antibody titers in neonates and infants decreased rapidly within 6 months after birth and then increased gradually with a maximum level at 7～14 years old. Although the titers of IgC antibodies specific to F and G glycoproteins fluctuated a little within the first 6 months of life , the absolute values were lower than that in old children. After 6 months the titers of antibodies to F and G glycoproteins rised gradually, especially the antibodies to F glycoprotein. The antihody titer reached its peak level at 3～7 year. of age for F glycoprotein and at 7 ～14 years for Gglycoprotein. From the correlative point of view , it suggests that RSV antibody correlates with F and Ci antibodies , but there is no correlation between F and G ancibodies.