LFA-1/ICAM-1单抗对ConA诱导脾淋巴细胞增殖及分泌细胞因子的影响

Effects of monoclonal antibody of LFA-1 /ICAM- 1 on the Con A induced proliferation and cytokine secretion of splenic lyrnphocytes

通过研究ＬＦＡ－１／ＩＣＡＭ－１单抗对ＣｏｎＡ诱导的脾淋巴细胞活化增殖及其分泌细胞因子的影响 ，探 讨了ＬＦＡ－１／ＩＣＡＭ－１分子在Ｔ细胞活化过程中所起的共刺激作用。结果表明，单独应用ＬＦＡ－１α链单抗（Ｍ１７／４．４．１１．９）或ＩＣＡＭ－１单抗（ＹＮ１／１．７．４）均不能引起脾淋巴细胞的增殖，但在加入ＣｏｎＡ诱导脾淋巴细胞增殖反应的最初８小时内加入ＬＦＡ－１单抗可以剂量依赖性地抑制ＣｏｎＡ诱导的脾淋巴细胞增殖反应及脾淋巴细胞分泌ＩＬ－２、ＩＰＮ－γ，而加入ＩＣＡＭ－１单抗却无此效应。说明ＬＰＡ－１在ＣｏｎＡ诱导的Ｔ细胞活化过程中起着重要的共刺激作用，ＬＰＡ－１通过与除ＩＣＡＭ－１以外的其它配体分子（如ＩＣＡＭ－３）相互识别，提供Ｔ细胞活化所必需的共刺激信号。

In order to further elucidate the role of LFA- I /ICAM -1 adhesion molecules on T cell activation , we investigated the effects of monoclonal antibody against LFA-1/ICAM - 1 on the Con A induced pruliferation and IL-2 ,IFN-r secretiun of splenic lymphocytes. Our results demonstrated that anti-LFA-1/ ICAM - I alone could not induced splenic lymphocyte proliferation. Adminstration of McAb of LPA- 1 in the first 8h after incubation showed a dose-dependent inhibitory effect on the Con A induced proliferation of splenic lymphocytes while McA b of ICAM- I showed no inhibitory effect. Anti-LFA-l cou M also inhibit Con A induced IL-2 ,IFN -Y secretion from splenic lymphocytes. These results suggested that LFA- 1 could exert its costimulatory function during Con A induced T cell activation through recognition with other receptor molecules other than ICAM- 1.