rhGM－CSF对狗骨髓辐射损伤治疗作用的定量病理研究

Quantitative pathological studies on hone marrow radiation injury treated with recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) in beagle dogs

目的；为了解细胞因子对骨髓辐射损伤的治疗作用。方法：用６０Ｃｏγ射线以５．５Ｇｙ照射１４只比格狗后，静脉注射低剂量（１０μｙ／ｋｇ·ｄ）及高剂量（２０μｇ／ｋｇ·ｄ）ｒｈＧＭ－ＣＳＦ，连续２０天，动物于照射后３０天活杀取胸骨，采用Ｈ·Ｅ及Ａｇ－ｎｏｒ嗜银蛋白染色和图像分析等技术，定量研究了ｒｈＧＭ－ＣＳＦ对骨髓造血的促进作用。结果表明：高剂量的ｒｈＧＭ－ＣＳＦ对狗骨髓辐射损伤后造血重建有明显的促进作用，即ｒｈＧＭ－ＣＳＦ能使骨髓中有核细胞数显著增多；能使增殖活跃的单核系细胞亦增多，与照射对照组比，差异均具有统计学意义（Ｐ＜０．０１或Ｐ＜０．０５）。讨论：高剂量ｒｈＧＭ－ＣＳＦ对狗骨髓造血功能的重建有促进作用。

By means of nuclear organizer region associated protein (Ag-nor) Staining and image analysis, we quantitatively studide the effect of rhGM-CSF on bone marrow hemopoiesis of 14 beagle dogse irradiated with 5 . 5Gy 60 Coγ-rays and treated with low (10μg/kg.d) and high (20μg/kg. d) dose of rhGM-CSF for 20 days. The dogs were sacrificed in 30 days after radiation to remove sternum. The results showed that high dose of rhGM-CSF obviously improved the reconstitution of bone marrow hemopoiesis. It may increase hemopoietic cells and monocyte lineages, and promote proliferation of these cells. All of these changes were compared with those of the controls, and the results indicate that the differences were significant ( P< 0 . 01 or< 0 . 05) . The authors hold that rhGM-CSF can be used to treat bone marrow radiation injury. The probable mechanism of rhGM-CSF therapy is discussed.