加热复合电离辐射对人骨髓细胞的影响

Effects of X-ray irradiation combined with hyperthermia on human bone marrow cells

目的：研究了加热与电离辐射后对人离体骨髓细胞存活率及形态等方面的影响。结果：骨髓细胞存活率随加热时间增加而呈直线下降，温度越高直线斜率越大。在形态学上，骨髓细胞受热后可出现非特异性退行性变化，随着时间的延长，这些变化更加明显。骨髓细胞受单纯Ｘ射线照射后，可见随着照射剂量的增大，细胞存活率明显下降。形态学上单纯３ＧｙＸ射线照射可使少数细胞出现空泡变性，线粒体肿胀，核膜破裂等变化。骨髓细胞受Ｘ射线照射后立即给予４２℃３０分钟的加热使骨髓细胞存活率下降的程度比各自单独作用之和要大（Ｐ＜０．０１）。电镜显示结构破坏明显，细胞支架系统、细胞器均受到破坏，细胞膜、核膜破坏乃至溶解，核浆、细胞浆外溢。

This paper reports on the effects of X-ray irradiation combined with hyperthermia on human bone marrow cells (BMC) in vitro. Observation was made on the morphology of treated cells under optic microscope and ultrastructural changes under electron microscope. The change was not obvious at first after treatment i, e, only the vacuolar degeneration was observed in a few cells under the EM. The survival of BMC alone after irradiation decreased with increase of the irradiation dose. The morphological changes included vacuolar degeneration of cells, swelling of mitochondria, and desintegration of nuclear membranes. The survival rate of BMC after irradiation combined with hyperthermia was significantly lower than that after treatment by either of them alone (P< 0 . 01 ). The morphological changes were as follows: the cell structure was destroyed, the cell support system and cell organelles were destroyed, the cell membrane and nuclear membranes were destroyed, and the cell plasma and nuclear sap overflowed .