细胞凋亡抑制基因Bcl－2对NIH3T3细胞增殖及辐射敏感性的影响

Effects of Bcl-2 gene transfer on proliferation and irradiation sensitivity of NIH3T3 cells

以含Ｂｃｌ－２的重组逆转录病毒转染包装细胞系ＰＡ３１７，经Ｇ４１８抗性筛选，获得单克隆细胞，扩增并收集培养上清感染ＮＩＨ３Ｔ３细胞，进而完成Ｂｃｌ－２基因向ＮＩＨ３Ｔ３细胞的导入。后者软琼脂集落形成率与细胞增殖速率均高于对照组，而辐射敏感性低于对照组。表明Ｂｃｌ－２基因对ＮＩＨ３Ｔ３细胞的增殖和辐射敏感性均具有一定的影响。

The packaging cell line PA317 was transfected with the recombinant retrovirus vector carrying Bcl-2.After 15 days, G-418-resistant clones were isolated and expanded, and the culture supernatant was collected to infect NIH 3 T 3.Compared with the control group, the infected NIH 3 T 3 cells were higher in agarose colony forming ability and proliferation and lower in irradiation sensitivity.