人皮肤慢性放射性溃疡c－fos、Rb蛋白异常高表达病理研究

A pathological study on overexpression of c-fos and Rb proteins in human radiation skin ulcer

作者应用ｃ－ｆｏｓ、Ｒｂ蛋白抗体和微波炉抗原修复法对人慢性放射性皮肤溃疡进行免疫组织化学研究。结果显示：①ｃ－ｆｏｓ蛋白在人放射性皮肤溃疡阳性率为８４．０％。阳性部位主要见于增生的鳞状上皮细胞核、增生的纤维母细胞以及小血管内皮细胞、小动脉中层平滑肌细胞核，部分细胞浆内有阳性反应。②将ｃ－ｆｏｓ蛋白在表皮的检出情况及反应强度与表皮的增生程度列表分析，提示ｃ－ｆｏｓ蛋白在各增生表皮以至癌变组织阳性率有升高趋势。③Ｒｂ蛋白在正常皮肤检测不出，在病变组织的增生部位均有表达且表达的阳性强度在各增生组织以至溃疡癌变组织内无明显差异，其阳性部位与ｃ－ｆｏｓ蛋白分布基本一致。以上结果说明ｃ－ｆｏｓ、Ｒｂ蛋白异常性高表达可能与放射性溃疡反复发作、经久不愈，最后癌变的机制等因素有关。

We performed an immunohistochemical study on human radiation skin ulcer by using antibodies against c-fos and Rb proteins and antigen-repairing method with a microwave oven.We found that the positive rates of overexpression of c-fos and Rb proteins were 84.0% and 100%,respectively.The overexpression of c-fos protein was mainly observed in cell nuclei of squamous epithelial cells,fibroblasts,endothelial cells,and leiomyocytes in media and fibrocytes in adventitia of arterioles.The location of the Rb protein overexpression was mostly similar to that of c-fos protein.The overexpression of c-fos and Rb proteins may be related to cancer transformation and poor healing of radiation-induced skin ulcers.