磷酸化蛋白质组学分析和定量技术的研究进展

Progress of Technology and Methodology in Qauntitative Phosphoproteomics

蛋白质的磷酸化是一种可逆性的蛋白质翻译后修饰,在生物体内起着极为重要的作用.近年来蛋白质翻译后修饰日益成为蛋白质组研究的热点之一.定量磷酸化蛋白质组学方法和技术的快速发展为研究蛋白质磷酸化时空动态变化,更好地了解生物学功能调节网络奠定了坚实的基础.作为蛋白质组学研究的一个重要组成部分,定量磷酸化蛋白质组学因其磷酸化蛋白质所具有的独特特征,在技术和方法研究方面将面临更为严峻的挑战.综述了磷酸化蛋白质组学定量的一些分析技术和方法的发展现状、优缺点以及未来的发展趋势.

Protein phosphorylation is one of the most important post-translational modifications. Reversible protein phosphorylation plays an essential role in regulation of cellular actions. Recently, phosphoproteomics is becoming a hotspot. Quantitative phosphoproteomics provides the possibilities to study temporal-spatial dynamics of protein phosphorylation and to better understand the regulatory networks of key processes in cells. As a part of proteomics, the quantitative phosphoproteomics faces more severe challenges since protein phosphorylation occupies high dynamics and extreme complexity. Advantages and disadvantages of several newly developed methods and technology were discussed, and the progress in quantitative phosphoproteome research realm was summarized.