拉萨地区乙型肝炎病毒携带者感染基因型的实验研究

Experimental study on the genotype of hepatitis B virus carrier in Lasa

【目的】研究拉萨地区乙型肝炎病毒携带者感染基因型的流行情况。【方法】采用聚合酶链反应(PCR)扩增乙型肝炎病毒S基因区,用末端标记方法对PCR产物标记并直接测序,测序结果和Gen Bank中的标准基因型序列相比较。【结果】60例标本中有46例可直接测序分型(76.7%),其中包括B型14例(少数民族5例)、C型12例(少数民族5例)、D型20例(少数民族3例);14例标本未能直接分型,考虑为混合型。【结论】拉萨地区HBV DNA基因型为B型、C型、D型和混合型,以D型为主,未发现A、E型。

[Objective] To investigate the main genotype of hepatitis B virus carrier in Lasa. [Methods] The S gene were amplified by PCR from the HBsAg positive patients in Tibet. After sequencing the end-labelled PCR production, the gene sequences were compared with the registered standard genotype sequences in GenBank. [Results] Of the 60 specimens, 46 （76.7%） could be detected directly, among them 14 were genotype B （5 were minority）, 12 were genotype C （5 were minority） and 20 were genotype D （3 were minority）. 14 specimens which undetected might be mixed genotype. [Conclusion] Genotype B, C, D and mixed type are detected in Lasa, the dominant genotype of HBV is D in this area, but genotype A and E are not found.