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大鼠不同组织的磷酸酶活性及钙调神经磷酸酶的钙依赖性 被引量:1

Study on the activity of phosphatases and the calcium dependence of calcineurin in different tissues of rat
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摘要 【目的】观察钙调神经磷酸酶(CaN)、非钙调神经磷酸酶和镁离子调控的蛋白磷酸酶(PP2C)在大鼠不同组织中的活性及分布,以及CaN在不同组织中钙依赖性的特点。【方法】取正常大鼠不同器官组织,分步离心提取蛋白,应用紫外分光光度法测定磷酸酶在大鼠各组织中的活性及CaN在不同组织中的钙依赖性特点。【结果】CaN在脑组织中活性最高、其次为骨骼肌组织和心肌组织,而在肝脏组织中活性最低(P<0.01)。非钙调神经磷酸酶在脑和肝脏组织中活性较高,而骨骼肌和心肌组织中活性较低(P<0.01)。PP2C活性在脑组织中最高,骨骼肌和心肌组织次之,在肝脏中活性低(P<0.01)。钙依赖性实验表明,改变钙离子浓度时,各个组织中CaN活性均呈钟型改变,钙离子浓度过高可抑制CaN活性。【结论】钙调神经磷酸酶、非钙调神经磷酸酶和PP2C在大鼠体内均有广泛分布,可能参与多种器官组织的功能调节;钙依赖性实验结果表明大鼠不同器官组织中CaN对钙依赖性有共性,高钙引起的CaN活性抑制可能与CaN的内源性抑制物质有关。 To observe the activity and distribution of phosphatases and the calcium dependence of ealcineurin in different tissues of rat. [Method] The activity of calcineurin and other phosphatases in different tissues, and the activity of calcineurin in different calcium with uv-spectrophotometer. [Results] The activity of calcineurin in brain was higher than that in other tissues (6.81 ± 2.41, P 〈 0.01 ) . The activity of phosphatases in brain was higher than that in other tissues (7.59 ±1.93, P 〈 0.01 ). The activity of PP2C in brain is higher than that in other tissues (4.55 ± 1.72, P 〈0.01). As the concentration of calcium was added, the activity of calcineurin was increased. When pCa increased to 4.52, however, the activity of calcineurin was decreased. [Conclusion] These results suggest that the activity of phosphatases in brain are higher than in other tissues, and the result of calcium dependence presents a change of a bell shape.
出处 《武警医学院学报》 CAS 2007年第2期157-159,共3页 Acta Academiae Medicinae CPAPF
基金 天津市教育委员会科研项目(200011)
关键词 组织 磷酸酶 钙依赖性 Tissues Phesphatase Calcium dependence
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参考文献9

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同被引文献17

  • 1陈雯,谢晓华,常连庆,李晓玲,刘宁,刘秀华.环孢霉素A抑制肾性高血压大鼠重要脏器钙调神经磷酸酶活化[J].心脏杂志,2006,18(1):39-42. 被引量:3
  • 2邱丽颖,余涓,陈崇宏.阿司匹林抗脑缺血/再灌注损伤的作用及机制[J].中国药理学通报,2006,22(8):972-976. 被引量:19
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