银杏叶提取物在大鼠肝移植缺血再灌注损伤中的预处理效用

Effect of extract of ginkgo biloba leaves on the precondition of liver graft in rat liver transplantation

目的:探讨银杏叶提取物对大鼠移植肝缺血再灌注损伤模型的预处理效用。方法:采用Kamada’s袖套法建立大鼠缺血再灌注原位肝移植模型。将大鼠随机分成假手术组(SO组)、生理盐水对照组(NS组)、银杏叶提取物预处理组(EGb组)。各组分别观察移植肝再灌注后2h、6h和24h肝组织中TNF-α、IL-1含量及血清ALT和AST含量和肝组织学变化。结果:SO组与NS组血清ALT和AST含量,肝组织TNF-α和IL-1活性明显升高(P<0.01),肝细胞形态学发生异常变化。EGb预处理组,上述指标的异常变化均明显减轻,与NS组比较差异有显著性意义(P<0.01)。结论:银杏叶提取物可通过抑制Kuffer氏细胞激活减少释放TNF-α和IL-1始动因子并调控缺血再灌注因子水平达到保护供肝作用。

Objective To explore the effect of extract of ginkgo biloba leaves on the precondition of liver graft in rat liver transplantation. Methods Male Sprague-Dawley rats were used as donors and recipients of orthotopic liver transplantation （ OLT ） , and were randomly divided into extract of gink cording go biloba leaves group （Egb） , NS control group （NS） , and sham operation group （SO） acto whether the extract of ginkgo biloba leaves was injected by the venous （40 mg/kg ） 1 h before the liver grafts harvesting. The rats were killed at 2 h, 6 h, and 24 h after the ischemia/ reperfusion. The serum concentrations of ALT and AST were determined and the liver tissue were sampled to observe the expression of TNF-α and IL-1. Results After the ischemia/reperfusion the concentration of ALT and AST and the expressions of TNF-α and IL-1 in the hepatic tissue in EGb group compared with the NS group （ P 〈 0.01 ） , and relieved the hepatocyte swelling and necrosis.Conclusion Ginkgo bilobo extract may decrease the release of TNF-α and IL-1 by inhibiting activation of kuffer cells and regulate the cell factors to protect the live.