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转基因拟南芥中外源基因的Southern blot检测 被引量：1

Southern Blot Analysis of Exogenous Transgenic Genes in Transgenic Arabidopsis Plants
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摘要 外源基因在转基因植物的稳定表达是获得理想转基因植物的首要条件。在我们进行的转基因植物研究中,根据转基因植物所带有的转基因成分和标记基因的DNA序列,采用Southern blot技术对转基因植物中的猪α-乳清蛋白基因和标记基因的拷贝数进行了分析。结果表明:外源基因的拷贝数和外源基因的稳定表达相关。 Abstract：The foreign genes are stably expressed in transgenic plant, which is main issue for plant transformation research. In this article, accoding to transgenic components and DNA sequence of marker genes in transgenic plant, we adopt southern blot technology to analyze porcine α -lactalbumin and copy numbers of marker genes in transgencic plant. The results showed the copynumber of exogenous genes is dramatically related to the stable expression of exogenous genes.
作者 芦春斌
机构地区 暨南大学生殖免疫中心
出处 《种子》 CSCD 北大核心 2007年第2期14-16,共3页 Seed
基金 教育部留学回国人员科研启动基金资助。
关键词 转基因植物 基因沉默 猪α-乳清蛋白基因 SOUTHERN BLOT分析 transgenic plants gene silencing porcine ot -lactalbumin southern blot
分类号 Q78 [生物学—分子生物学]
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参考文献13

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  • 9芦春斌.转基因植物(农产品)中转基因成分的PCR检测[J].种子,2006,25(1):1-3. 被引量：1
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二级参考文献15

  • 1芦春斌.植物化的猪α-乳清蛋白基因人工合成[J].种子,2004,23(12):3-5. 被引量：2
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  • 4Trick FIN, Dikins ER, Santaren ED et al. Recent advances in soybean transformation. Plant Tiss. Cult. Biotechnol. 1997.3:9-26.
  • 5Maughan PJ, Philip R, Cho MJ et al. Bio]istic transformation,expression and inheritance of Bovine Beta-Casein in Soybean. In Vitro Cell Dev. Bilo. -plant, 1999,35:344 - 349.
  • 6Yang SH, Moran DL, Jia HW et al. Expression of a synthetic porcine α- lactalbumin gene in the kernels of transgenic maize. Transgenic Research, 2002,11 : 11 - 20.
  • 7Paula Elomaa,Yrj? Helariutta,Mika Kotilainen,Teemu H. Teeri,Robert J. Griesbach,Pauli Sepp?nen. Transgene inactivation inPetunia hybrida is influenced by the properties of the foreign gene[J] 1995,Mgg Molecular & General Genetics(6):649～656
  • 8Fran?ois Dorlhac,Michel Vincentz,Yves Chupeau,Hervé Vaucheret. Co-suppression of nitrate reductase host genes and transgenes in transgenic tobacco plants[J] 1994,MGG Molecular & General Genetics(6):613～621
  • 9P. Meyer,I. Heidmann. Epigenetic variants of a transgenic petunia line show hypermethylation in transgene DNA: an indication for specific recognition of foreign DNA in transgenic plants[J] 1994,MGG Molecular & General Genetics(4):390～399
  • 10A. J. M. Matzke,F. Neuhuber,Y. D. Park,P. F. Ambros,M. A. Matzke. Homology-dependent gene silencing in transgenic plants: epistatic silencing loci contain multiple copies of methylated transgenes[J] 1994,MGG Molecular & General Genetics(3):219～229

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种子
2007年 第2期

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