应用基因表达谱芯片技术筛选GABA作用下肝星状细胞差异表达基因

Screening of genes differentially expressed in hepatic stellate cells acted by gamma aminobutyric acid with microarray assay

目的:应用基因芯片技术检测γ-氨基丁酸(GABA)对肝星状细胞(HSC-T6)基因表达谱的影响.方法:10μmol/L的GABA作用于HSC-T6细胞48 h,提取mRNA,逆转录为cDNA.双PBS作用的HSC-T6细胞为对照,进行cDNA芯片分析.结果:在4096个基因表达谱的筛选中,发现有11个基因表达水平显著上调,26个基因表达水平显著下调.结论:成功地应用基因芯片筛选GABA作用下肝星状细胞差异表达基因,证明GABA对肝星状细胞的表达谱有显著作用.

AIM： To screen genes regulated by gamma aminobutyric acid （GABA） in hepatic stellate cells （HSC） using microarray assay, and to investigate GABA biological function. METHODS： HSC-T6 was co-cultured with 100 μmol/L GABA for 48 hours, and then mRNA was extracted from the cells to reversely transcribe into cDNA. HSC-T6 co-cultured with phosphate buffer saline （PBS） was used as controis. The cDNA profile was analyzed by microarray assay. RESULTS： For the profile of 4096 genes, 37 genes were found differentially expressed, of which 11 were up-regulated while 26 were down-regulated signifcantly. CONCLUSION： Differentially expressed genes under the action of GABA are successfully screened by microarray technique, demonstrating that GABA can affect the gene profile of HSC.