针刺不同穴位对不同应激模型大鼠迟发反应期IL-2影响的实验观察

Influence of needling in different acupoints on IL-2 Level of delayed reaction period in different stress rat models

目的通过观察针刺、束缚和致病因子对白细胞介素-2(IL-2)水平变化的影响及其作用规律,探明大鼠免疫功能失调状态下IL-2水平变化的特点,以揭示针刺免疫调控的机理。方法以佐剂性关节炎(AA)和束缚应激模型作为研究对象,对任脉六穴和关元穴进行电针刺激,采用放免法测定血清IL-2水平。结果与正常组比较,AA模型组、束缚模型组血清IL-2含量呈增高趋势,无显著性差异;与AA模型组、束缚模型组比较,AA六穴组、束缚六穴组和AA关元组、束缚关元组血清IL-2含量均有升高,AA关元组呈显著性差异(P<0.01),束缚关元组呈显著性差异(P<0.05)。结论针刺可上调AA模型大鼠、束缚模型大鼠血清IL-2的水平,对机体的免疫功能进行调节;关元穴的作用明显。

Purpose To study the change characteristics of interleukin 2 （IL-2） level in rats with immunity disorder and reveal the mechanism of immunomodulation by acupuncture through the observation on the influence and effective law of acupuncture, bondage and pathogenic factors on IL-2 level changes. Methods The electroacupuncture was used in six acupoints of Conception Meridian and Guanyuan （CV4） and the serum IL-2 level was determined by radioimmunoassay in the adjuvant arthritis （AA） model and bondage stress model. Results The IL-2 level had an increasing tend in AA model group and bondage model group compared with that in the normal group, but the difference was not significant （P 〉0.05 ）. The IL-2 levels were all increased in AA model group with six acupoints, AA model group with CV4, bondage model group with six acupoints and bondage model group with CV4 compared with those in AA model group and bondage model group. The difference was significant in AA CV4 group （P 〈 0.01 ） and too in bondage CV4 group （P 〈 0.05 ）. Conclusions Acupuncture can up-regulate IL-2 level in rats of AA model and bondage model, and regulate the body immunity. The acupuncture in CV4 has more effective result.