摘要
本文通过摇瓶实验确定了自选青霉菌株Z—I—C分批发酵生产葡萄糖氧化酶(GOD)的适宜工艺条件,并在2.6立升台式发酵罐中进行了小试,监测了发酵过程中溶氧(DO)、pH、还原糖、菌体干重(DCW)、氨基氮(NH2-N)和酶活等工艺参数的变化规律,为进一步提高GOD的发酵产率提供了依据。
In this paper.Glucose Oxidase fermentation process was studied by shaked culture.Based on some outstanding processes.the maximum enzyme activity is 96.7 u/ml in 2.6L fermentor which is higher than that of the national fermentation.