联合检测血清透明质酸与层粘连蛋白对肝纤维化的诊断价值

Diagnostic Value of Liver Fibrosis with Combination Detection of Serum Hyaluronic Acid and Laminin

目的探讨血清透明质酸(HA)与层粘连蛋白(LN)对肝纤维化的诊断价值。方法用干燥试管取135例正常人、急慢性肝炎及肝纤维化病人新鲜静脉血,离心后取血清按试剂盒说明书的操作步骤处理,再吸弃上清,用γ计数仪测沉淀物放射性,转换成HA及LN浓度,并对HA及LN高的病人采用肝穿刺活检,对HA及LN结果与肝穿刺结果作对照分析。结果HA及LN分别以84μg/L和133μg/L为阳性界值。其中HA敏感性较高,特异性较LN低;LN敏感性较HA低,但其特异性较HA高,以两者联合检测为最优。HA与LN的敏感度分别为86.4%和81.8%,特异度分别为84.9%和94.6%。结论HA与LN对肝纤维化具较好的诊断价值。两者联合检测可显著提高其敏感度及特异度,且与肝穿刺活检结果基本一致。

Objective To discuss the diagnostic value of liver fibrosis with combination detection of serum HA and LN, methods： The serum of 135 cases of normal adult, acute and chronic hepatitis, and liver fibrosis patient was detected with HA and LN and compared with the biopsy of LF. The control analysis was performed. Results The positive value was 84μg/L of HA and 133μg/L of LN, The sensitivity of HA was higher than LN and specificity was varied, The sen- sitivity was 86.4 % of HA and 81,8 % of LN, The specificity was 84.9 % of HA and 94.6%, Conclusion The results showed that the detection of HA and LN should be valuable for LF. The combination detection of HA and LN might be as same as the result of biopsy of LF.