鸡马立克病病毒814株gE、gI、gp82基因的克隆及序列分析

Cloning and Sequence Analyses of gE、gI、gp82 Gene of Marek's disease virus Strain 814

从感染鸡马立克病病毒血清Ⅰ型(MDVⅠ)814株的鸡胚成纤维细胞(CEF)中提取病毒总DNA,以其为模板,根据GenBank中MDVⅠGA株基因组gE、gI、gp82基因序列,设计并合成3对特异性引物,用PCR方法分别扩增了814株的gE、gI、gp82基因,并将扩增的基因片段克隆到pMD18-T载体中,进行序列测定,应用DNA Star软件分析814株gE、gI、gp82基因核苷酸序列,并与已发表的MDVⅠ毒株序列进行了比较。结果表明,不同MDVⅠ毒株的gE、gI、gp82基因同源性很高,814株与已发表的MDVⅠ毒株的gE、gI、gp82基因核苷酸序列同源性分别在99.4%、98.9%和99.6%以上。

Three sets of PCR primers were designed according to the gE, gI, gp82 genes of genome sequence of strain GA of Marek＇s disease virus serotype-l（MDV Ⅰ ）. The three genes of strain 814 of MDVⅠ were amplified from CEF infected with strain 814 of MDV I , and the PCR products were cloned into pMD18-T vector, respectively. The genes were sequenced. Their nucleotide sequences were analyzed by DNAStar software. Results indicated that homologies of genes gE,gI,gp82 are more than 99.4 %, 98.9 %, 99.6 %, respectively between strain 814 and other strains of MDV Ⅰ.