摘要
目的:阐明油茶皂苷(SQS)所产生心肌保护作用与AE3蛋白的基因表达关系,方法:采用RNA i技术,在心肌细胞水平上建立AE3蛋白低表达、同时建立缺氧/复氧损伤(A/R)模型及SQS预适应模型;通过测定,心肌细胞的博动频律、细胞生存率以及细胞培养液中LDH的变化;同时检测心肌细胞中AE3 mRNA、AE3蛋白的表达变化,结果:SQS预处理能明显提高A/R损伤后细胞存活率,减少LDH的漏出,AE3 mRNA、AE3蛋白表达明显上调,与A/R组相比均有显著性差异(均p<0.01);结论:在心肌细胞中转导入三段有AE3基因序列特异性的dsRNA后,可引起AE3 mRNA及蛋白低表达,同时取消SQS预处理的心肌保护作用.为其进一步的新药开发提供理论与实验依据.
Objective ： indicating the relationship between the pretection function of cardiac muscles produced by sasanquqsaponin （SQS） and gene expression of AE3 protein. Methods： taking RNAi techniques, establishing AE3 protein low expression on the level of cardiac cells, as well as establishing the model of abscent oxygen/repair oxygen and the pre -adapted model of SQS; through different intervention, assaying the changes of throb frequency of cardiac muscles cell. existing rate of cells and LDH in the cell of cultivating fluid; meantime, assaying the expressing changes of AE3 mRNA. AE3 protein in the cardiac muscles cells. Results： pretreatment of SQS can improve livability of A/R damaged cells; reduce the leak of LDH; Protein expression of AE3 mRNA. AE3 are upgraded apparently. Comparing to group A/R, there is apparent change （all p 〈0. 01） ; Conclusion： conduct three sects dsRNA with the specialty of AE3 gene series into the cardiac cells, there will cause AE3 mRNA and protein low expression; meantime cancel the cardiac protection function of SQS pretreatment. In order to provide theories and experimental gist for further new drugs development.
出处
《宜春学院学报》
2006年第6期12-14,共3页
Journal of Yichun University
基金
江西省教育厅自然科学课题
关键词
油茶皂甙
A/R
预处理
RNAI技术
心肌细胞
Sasanquqsaponin （SQS）
A/R damage
Pretreatment
RNAi techniques
Cardiac muscles cells
