烹调油烟冷凝物诱发支气管上皮细胞HPRT基因位点突变的研究

Study on Frequency of HPRT Locus Mutation in Human Bronchial Epithelial Cells Induced by COFC

目的研究烹调油烟冷凝物致人支气管上皮细胞HPRT基因位点突变的影响及二甲基亚砜的拮抗作用,以探讨烹调油烟的致突变性及其防护。方法采用体外多核细胞法研究烹调油烟冷凝物对人支气管上皮细胞HPRT基因位点的影响及二甲基亚砜的防护作用。结果烹调油烟冷凝物诱发人支气管上皮细胞HPRT基因位点突变,且突变频率随着染毒浓度的增加而升高,突变频率与烹调油烟冷凝物浓度之间存在一定的剂量-效应关系,二甲基亚砜对HPRT基因位点突变有显著的拮抗作用。结论烹调油烟冷凝物能诱发人支气管上皮细胞HPRT基因位点突变,二甲基亚砜能抑制烹调油烟冷凝物的诱变作用。HPRT基因突变频率可望成为接触环境致突变物人群检测的敏感指标。

Objective To study the mutational effect of COFC on HPRT gene of human bronchial epithelial cells and protection of DMSO. Methods Cytokinesis- block （CB） assay was used to detect the frequency of HPRT locus mutation induced by COFC and its dose - effect relationship and protection of DMSO. Results The test showed that COFC could induce HPRT locus mutation in human bronchial epithelial and there was a linear relationship between the concentration of COFC and the mutation frequency （MF} of HPRT gene. The mutation could be inhibited effectively by DMSO. Conclusion COFC may result in HPRT locus mutation in human bronchial epithelial cells. It is expected that this assay might be used to study the environmental exposed population in future. DMSO can inhibit the mutation induced by COFC.